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open reading frame encoding icre  (Addgene inc)


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    Addgene inc open reading frame encoding icre
    Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted <t>open</t> <t>reading</t> <t>frame</t> (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .
    Open Reading Frame Encoding Icre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/open+reading+frame+encoding+icre/pmc12800110-193-1-24?v=Addgene+inc
    Average 92 stars, based on 7 article reviews
    open reading frame encoding icre - by Bioz Stars, 2026-06
    92/100 stars

    Images

    1) Product Images from "Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia"

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia

    Journal: Scientific Reports

    doi: 10.1038/s41598-025-31077-z

    Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted open reading frame (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .
    Figure Legend Snippet: Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted open reading frame (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .

    Techniques Used: Injection, Expressing



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    Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted <t>open</t> <t>reading</t> <t>frame</t> (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .
    Open Reading Frame Encoding Icre, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    open reading frame encoding icre  (Addgene inc)
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    Addgene inc open reading frame encoding icre
    Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted <t>open</t> <t>reading</t> <t>frame</t> (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .
    Open Reading Frame Encoding Icre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1 article reviews
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    Image Search Results


    Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted open reading frame (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .

    Journal: Scientific Reports

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia

    doi: 10.1038/s41598-025-31077-z

    Figure Lengend Snippet: Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted open reading frame (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .

    Article Snippet: The open-reading-frame encoding iCre (based on pBSII-iCre , was amplified by PCR with linkered primers and inserted the BamHI and EcoRI sites of pOTTC293 (Addgene; #60057) using ligation-independent cloning (In-Fusion; Clontech).

    Techniques: Injection, Expressing

    Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted open reading frame (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .

    Journal: Scientific Reports

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia

    doi: 10.1038/s41598-025-31077-z

    Figure Lengend Snippet: Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted open reading frame (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .

    Article Snippet: The open-reading-frame encoding iCre (based on pBSII-iCre , was amplified by PCR with linkered primers and inserted the BamHI and EcoRI sites of pOTTC293 (Addgene; #60057) using ligation-independent cloning (In-Fusion; Clontech).

    Techniques: Injection, Expressing